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Abstract
HAWKINS, R. B., TOLMACH, L. J., AND GRIFFITHS, T. D. DNA Synthesis in Generation 1 in X-Irradiated HeLa Cells. Radiat. Res. 85, 85-98 (1981). Measurements of DNA replication in a line of HeLa S3 cells during the generation (Generation 1) following that in which the cells are irradiated with 500 rad of 220-kV X rays (Generation 0) were carried out according to a number of different experimental protocols. These involved preirradiation labeling of the cells with low levels of ['4C]thymidine in Generation - 1 to provide a measure of the template DNA, synchronization by mitotic collection in Generation 0, resynchronization by either mitotic recollection or temporary drug-induced blockades in Generation 1, and either labeled-thymidine incorporation or density-label transfer during Generation 1. The results show that those cells that progress through S phase of Generation 1 and divide at the next mitosis replicate a full complement of DNA, within the approximately 10% uncertainty of the experimental methods. However, apparent deficits of as much as 45% are measured if resynchronization in Generation 1 is effected by drug treatment following manipulations of the culture in the presence of particular media and drugs during Generation 0. These are attributed to combined radiation- and drug-induced disturbances in cell progression.
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