Abstract
DNA synthesis in different stages of the male meiotic prophase of the rat was studied by autoradiography after incubation of small segments of isolated seminiferous tubules with tritiated thymidine. Compared to incubation of cell suspensions, this technique gives the advantage that the physiological contacts between different cells are preserved, which diminishes the damage of the cells and their DNA. In addition, the recognition accuracy of the meiotic stages is increased as the morphology of the developing acrosomes can be used as an additional criterium for identification of the stages of the spermatogenic epithelial cycle. Highest synthetic rate was found in the mid-pachytene spermatocytes, while moderate or low rate was observed in leptotene, zygotene, early pachytene, late pachytene and diplotene stages.
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