Abstract
Solid-state nanopore DNA sequencing modified method is developed. Method is based on the tunnel current investigation through the nanogap made on lateral gold electrodes in the form of nanowires or nanoribbons. The movement of DNA in aqueous solution is regulated by the potential applied to reference electrode. The potential applied to the lateral metal electrodes helps to the creation of the molecular junctions. They consist of the nucleosides passing through the pores. Taking into account that DNA moves under gravity, electrophoretic and drag forces, the analytic expression for the DNA translocation speed is calculated and analyzed. The conditions for decreasing the DNA translocation speed or increasing the nucleosides reading time are received. It is shown that one can control value of the DNA molecules bases reading time and the frequency of the bases passes by the choice of magnitude of the potential applied to reference electrode. Our results, therefore potentially suggest a realistic, inherently design-specific, high-throughput nanopore DNA sequencing device/cell as a de-novo alternative to the existing methods.
Highlights
The process of DNA sequencing is a precise determination of the amount and distribution of the nucleotides in DNA molecules
The movement of DNA in aqueous solution is regulated by the potential applied to reference electrode
It is shown that one can control value of the DNA molecules bases reading time and the frequency of the bases passes by the choice of magnitude of the potential applied to reference electrode
Summary
The process of DNA sequencing is a precise determination of the amount and distribution of the nucleotides in DNA molecules. One major challenge of nanopore-based DNA sequencing technology is to find an efficient way to reduce DNA translocation speed. A key challenge to DNA sequencing with nanopores is to find methods to slow down and control DNA translocation. DNA translocation speeds can be reduced somewhat by decreasing temperature [12] [13], or increasing solvent viscosity [7] [14], but these methods do not reduce the variations in the translocation dynamics because of DNA-pore interactions [15] [16] [17] [18]. In this paper a modified architecture/design for measuring cell of DNA sequencing using tunneling current is offered in order to control and optimize the translocation speed of the DNA molecules. We study the features of the dynamics of the movement of DNA molecule in an aqueous solution under gravity, electrophoretic and drag forces in order to reduce the rate of movement of the DNA and increase DNA reading time
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