Abstract
T3 and T7 phages package recombinant plasmids carrying DNA necessary for DNA packaging (thepacsequences) of T3 and T7, respectively. Packaging is specific between T3 and T7. Thepacsequence has a bipartite structure, consisting of target sequences for processing of concatemeric DNA (pacC) and its left side flanking sequence containing a promoter for phage RNA polymerase (pacB). To determine the sequences responsible for the specificity of plasmid DNA packaging, plasmids chimeric for thepacB andpacC sequences of T3 and T7 were constructed. Analysis of packaging of the chimeric plasmid DNAs showed thatpacB is responsible for the packaging specificity of T3 and T7 DNAs. Plasmids carrying the genetic right end of T3 and T7 DNA interfered with the growth of T3 and T7 phages, respectively. Interference was specific between T3 and T7.pacB and sequences betweenpacB andpacC, but notpacC, were responsible for the interference. The specificity of interference was determined bypacB and sequences responsible for interference were partially defined.
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