DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Abstract

In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank (OP480437-39). Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94% 27.26 A; 26.78%-26.84% T; 28.30-28.62% G; 17.39-17.92% C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement (0%) between the Eustrongylides excisus isolates identified in this study (OP480437-39) and the Eustrongylides excisus isolate registered in the GenBank (MK007967, MT415236, MK545494).The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer (ITS) region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.