DNA Restriction Fragment Length Polymorphisms AmongMycosphaerella graminicola(AnamorphSeptoria tritici) Isolates Collected from a Single Wheat Field

Abstract

Restriction fragment length polymorphism (RFLP) markes were used to measure the amount and distribution of genetic variation in a fungal pathogen population on a microgeographical scale. Ninety-three isolates of Septoria tritici sampled from a single wheat field were assayed for RFLP variation using eight probes that hybridized to single RFLP loci and one probe that hybridized to two RFLP loci in nuclear DNA. Single locus and multilocus analysis of RFLP data indicated that a high level of genetic variability was distributed on a fine scale within this population (...)