Abstract
At the completion of SV40 DNA replication the two newly replicated daughter molecules segregate, producing two circular intermediates that sediment at 16 S. One or both of these segregants contain a single-stranded region or gap, bounded by a 3′-hydroxyl and 5′-phosphate group. Treatment of these intermediates in vitro with T4 DNA polymerase and E. coli polynucleotide ligase repairs this gap and produces a closed circular molecule. The position of this gap or single-stranded region has been determined with the aid of restriction enzymes (Eco R 1, HpaII) and was found to be at or very close to the terminus of DNA replication (about 0.15 units). These results suggest that the segregation of the two interlocked daughter molecules of SV40 DNA occurs before DNA replication is complete, that is, before the last phosphodiester bonds have been synthesized.
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