Abstract
The rate of [ 3H]-thymidine incorporation into DNA and the thymidine kinase activity were measured in salivary glands of Rhynchosciara angelae throughout the last part of larval life. The values vary in parallel rising to a maximum at the time of appearance of giant chromosomal puffs and then falling. [ 3H]-Bromodeoxyuridine is incorporated into salivary gland DNA. However, only after shearing to low molecular weight (1.5 × 10 6 dalton) does this DNA give rise to a heavy band upon CsCl centrifugation. This fact suggests a low rate of DNA chain elongation at the polytene chromosomes.
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