Abstract
The fate of 3H-dTTP incorporated into DNA in isolated S phase nuclei from Chinese Hamster Ovary cells was examined. 3H-dTTP observed in 4 S DNA subunits after a pulse-label becomes acid-soluble during a chase performed under conditions which permit continued active DNA synthesis. 3H-dTTP incorporated into longer DNA subunits is not affected by these chase conditions. This selective loss of 4 S pulse-label requires active DNA synthesis. In incubations which do not permit continued DNA synthesis, either there is little loss of label or the loss occurs equally from the 4 S and larger DNAs. Possible reasons for a metabolically active 4 S subunit are discussed.
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