DNA regions supporting hippocalcin gene expression in cell lines

Abstract

The rat hippocalcin gene −3.2 to +0.6 kb region activates reporter gene expression in the NG108-15 and PC12 neuronal cell lines, but not in NIH3T3 or HEK-293 cells. Three fragments (−3.2 to −2.6, −2.6 to −2.3 and −2.3 to −1.8 kb) weakly activate transcription, and "−1.8 to −1.5" kb is a strong activator. Thus cell type-specific expression of the rat hippocalcin gene is regulated by distributed elements in the −3.2 to −1.5 kb region.