DNA purification and isolation of genomic DNA from bacterial species by plasmid purification system

Abstract

We have successfully adapted the Wizard® MagneSil™ plasmid DNA purification system to isolate genomic DNA from soil bacteria strains important in research and the agricultural industry. In this work, we describe the modified protocol for isolating genomic DNA from soil bacteria using manual and automated approaches on the Biomek® 2000. The isolated genomic DNA was then used to PCR amplify an 875 bp DNA fragment. We have developed a simplified protocol for the isolation and purification of PCR quality genomic DNA from soil bacteria using Wizard® MagneSil™ RED. Yields of genomic DNA ranged from 10 to 100+ng. The experiments here outline both the manual and automated procedures and demonstrate robust PCR product amplification from minimal volume amounts of final eluate. We concluded that the plasmid purification system is a high quality, and suitable for molecular analyses such as PCR, restriction enzyme. Key words: DNA, plasmid purification, PCR amplification.