Abstract
In the presence of sufficient DNA (from biological stains) on a binding substrate, multiplex PCR can be carried out in-situ without an extraction step. In this study, blood dried on six different fabrics, namely, cotton, rayon, nylon, wool, acrylic and polyester were tested. Multiplex PCR using the AmpF lSTR Identifiler kit on a 2- to 3-mm square bloodstain successfully amplified the DNA from cotton, rayon, nylon and wool. A partial profile was obtained from the bloodstain on acrylic. No profile was obtained from the bloodstain on polyester. The binding properties of these various fabrics with DNA were investigated in this study.
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