Abstract
In silico sequence diversities of four orthologous plant gsh1 genes and their anino acid translates of GSH1 proteins (Glutathione Synthase) were compared to the non-orthologous prokaryotic gshI/GSHI gene/protein of E. coli (NCBI # X03954). Primer pair was designed and transgene detection was carried out in two types of gshI-transgenic poplar clones (Populus x canescens) of ggs11 (cyt-ECS) and lgl6 (chl-ECS). Usefulness of genetic modification technologies (GMO) is indicated.
Highlights
Transgenes represent genetic markers artificially introduced in laboratory motivated to improve crops
The ability to select and identify desired genotypes in cells, tissues or intact plants laid the fundamentals for application of genetic transformation of plants and animals by tools of biotechnology
Multiple sequence alignments for primer design Nucleotide sequences of genes gsh1 were downloaded from the National Center for Biotechnology Information (NCBI) databases [30]
Summary
Transgenes represent genetic markers artificially introduced in laboratory motivated to improve crops. Detection of marker in the Genetically Modified Organism (GMO), and its vegetative or sexual progenies; and monitoring it in test and cultivated populations as well as in exposed non-target cross-pollinated populations is of fundamental and practical importance. The genetically modified state of an organism, i.e. the presence of the transgene, is verified essentially by DNA profiling. Selecting the DNA sequence for DNA profile is straightforward because a known sequence is introduced. Introduction of genes, self or foreign, into plants had prerequisites. The ability to select and identify desired genotypes in cells, tissues or intact plants laid the fundamentals for application of genetic transformation of plants and animals by tools of biotechnology
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