Abstract
The aim of this study is to compare the efficiency of different validated methods for DNA extraction using commercial kits. DNA extraction was performed on fresh liquid blood, old bloodstains, cigarettes butts, semen stains and hairs. Samples were quantified by the AluQuant Human DNA Quantitation System. Amplification was carried out by GeneAmp 2400 and 9700 Thermal Cyclers using the AmpFlSTR Identifiler kit. Amplified products were analyzed into both ABI PRISM 310 and 3100 Genetic Analyzers employing ABI softwares.
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