DNA patterning utilizing mixed monolayer template by phase separation between hydrocarbons and fluorocarbons and evaluation of second-order structural maintenance ability

Abstract

Adsorption of DNA molecules on mixed phase-separated monolayers composed of a hydrocarbon-containing comb copolymer with an s-triazine ring and a fluorinated s-triazine derivative and their fluorescence emission behavior were investigated. DNA molecules derived from salmon testes were selectively adsorbed only on the diamino-s-triazine ring of the hydrogenated copolymer in the monolayer from the subphase. A mixed monolayer of a hydrocarbon-based comb copolymer and an s-triazine derivative with fluorocarbons exhibited phase separation behavior on water surfaces and indicated expansion behavior, reflecting interactions on the subphase containing DNA molecules. The phase-separated morphology changed depending on the mixing ratio of the hydrogenated copolymer and fluorinated s-triazine derivative. After DNA adsorption, the height of the domain formed by the hydrocarbon chain was selectively increased. In addition, because the DNA molecules adsorbed on the phase-separated template film maintain their fluorescence emission ability, the second-order structure, which is the origin of the emission ability, was evaluated using circular dichroism spectroscopy. The phase-separated, template-adsorbed DNA molecules maintained the same positive Cotton effect as in solution and were predicted to retain exquisite and delicate steric structure, leading to functional occurrence.