Abstract
Self-assembled DNA nanostructures have recently emerged as a type of drug delivery carriers due to their suitable sizes, well-defined nanoscale shapes, precise spatial addressability, and excellent biocompatibility. Here, we describe practical procedures in detail for the design and construction of DNA nanostructures with different width and patterns by long rolling circle amplification (RCA) strands and a few short staples, and provide practical guidance and troubleshooting advice for delivering CpG immunostimulatory drugs with these RCA based DNA nanostructures.
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