Abstract
The Layer-by-Layer (LbL) method using polyelectrolytes is a typical strategy for preparing functional polymeric multi-layered films. Besides the electrostatic force between positively and negatively charged polyelectrolytes, the hybridization force between complementary DNA strands can be also used for building the DNA LbL films. Herein, we report the thermal responsible DNA LbL film that is designed to release specific DNA oligomers (10 bases) at an elevated temperature. Because the temperature and the ionic strength (IS) of the medium affect the hydrogen bonds in the double stranded DNA, the structural stability of the film against temperature and IS was investigated. The loading and release of the releasable oligomers were repeatedly performed on the DNA LbL film.
Highlights
The Layer-by-Layer (LbL) method was developed for the formation of thin polymer films by alternatively adsorbing positively and negatively charged polyelectrolytes on a charged surface [1]
Because the additional sequences not for forming the layered structure can be inserted in the building blocks, the functions such as the programmed degradation through competitive hybridization [14], the postcrosslinkable regions to increase the film stability [11], and the sequence to detect specific materials [15-17] can be introduced within the film
The changes in the frequency of the quartz crystal microbalance (QCM) during the film depositions using the type I strands or the type II stands without Y were observed
Summary
The Layer-by-Layer (LbL) method was developed for the formation of thin polymer films by alternatively adsorbing positively and negatively charged polyelectrolytes on a charged surface [1]. With the aid of the template-assisted method, the LbL method can be applied for preparing functional polymeric capsules for smart drug carriers [2,3,4]. Analogous to the use of positively and negatively charged materials as building blocks in the LbL films [1, 2], the DNA LbL films are constructed from complementary pairs of oligonucleotides [ 7 - 14 ]. The physicochemical properties of the films and the hollow capsules made by the DNA LbL assembly have been investigated, and it was found that the properties can be controlled by the sequences of the DNA building blocks. Because the additional sequences not for forming the layered structure can be inserted in the building blocks, the functions such as the programmed degradation through competitive hybridization [14], the postcrosslinkable regions to increase the film stability [11], and the sequence to detect specific materials [15-17] can be introduced within the film
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