DNA-modified boron-doped diamond electrode as a simple electrochemical platform for detection of damage to DNA by antihypertensive amlodipine

Abstract

The proposed paper focuses on the development of a simple electrochemical platform based on the immobilization of double-stranded DNA (dsDNA) at lab-made boron-doped diamond electrode (BDDE) to explore DNA damage by antihypertensive amlodipine. Two ways of immobilization such as simple drop-casting approach and potentiostatic accumulation were investigated. The potentiostatic accumulation using 300 mV for 10 min was selected in further experiments owing to substantial flattening and decrease of the current response of [Fe(CN)6]3−/4− redox indicator when compared to drop-casting procedure. Such prepared DNA-modified BDDEs were incubated in the presence of antihypertensive amlodipine at various incubation times to examine potential damage to DNA. The results revealed the greater damage to dsDNA leading to its breaking, which was evidenced by relative value of anodic current peak (ΔI rel) varying from 44.4 to 14.4 % as incubation time increased from 5 to 20 min. The proposed platform using BDD as an electrode substrate for the DNA immobilization could represent the perspective electrochemical tool for monitoring of DNA damage in the presence of various structurally and biologically active compounds.