DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings

Abstract

In the current study, Arabidopsis seedlings were hydroponically grown on MS media containing cadmium (Cd) of 0–2.0 mg L −1 for 60 h of treatment. Gene expression profiles were used to relate exposure to Cd with some altered biological responses and/or specific growth effects. RT-PCR analysis was used to quantitate mRNA expression for seven genes known to be involved in DNA mismatch repair (MMR) system and cell division. Results indicated that Cd concentrations of 0.25–2.0 mg L −1 cause increased total soluble protein levels in shoots of Arabidopsis seedlings in an inverted U-shaped dose–response manner. Exposure to 0.25 and 0.5 mg L −1 of Cd dramatically induced expression of four genes (i.e. proliferating cell nuclear antigen 2 (at PCNA 2), MutL1 homolog (at MLH1), MutS 2 homolog (at MSH2) and at MSH3) and five genes (i.e. at PCNA1,2, at MLH1 and at MSH2,7), respectively, in shoots of Arabidopsis seedlings; Exposure to 1.0 mg L −1 of Cd significantly elevated expression of only two genes (at MSH6,7), but caused prominent inhibition in expression of three genes (at PCNA2, at MLH1 and at MSH3) in shoots of Arabidopsis seedlings. The expression alterations of the above genes were independent of any biological effects such as survival, fresh weight and chlorophyll level of shoots. However, shoots of Arabidopsis seedlings exposed to 2.0 mg L −1 of Cd exhibited statistically prominent repression in expression of these seven genes, and showed incipient reduction of fresh weight and chlorophyll level. This research provides data concerning sensitivity of expression profiles of at MLH1, at MSH2,3,6,7 and at PCNA1,2 genes in Arabidopsis seedlings to Cd exposure, as well as the potential use of these gene expression patterns as representative molecular biomarkers indicative of Cd exposure and related biological effects.