Abstract
Pathogenesis of the endometriosis is complex and the etiology is still unclear. The objective of this study was to examine that endometrial gene expression in late secretory phase endometrium differs between patients with and without endometriosis. Five patients with proven advanced-stage endometriosis and 5 controls underwent endometrial biopsy in the late secretory phase. Analysis of eutopic endometrial gene expression was performed using Affymetrix gene arrays and differentially expressed genes were assigned to gene ontology groups based on overrepresented analysis using Database for Annotation, Visualization, and Integrated Discovery software. Four hundred sixty two genes were identified as up-regulated such as matrix metalloproteinase 10, cytochrome P450 family 24 subfamily A polypeptide 1, matrix metalloproteinase 3, chemokine (C-C motif) ligand 20, Rho family GTPase 1, interleukin 1-beta, and insulin-like growth factor binding protein 1. Six hundred forty three genes were down-regulated in all endometriotic samples. A lot of genes related with metabolic process, cellular ketone metabolic process and ncRNA metabolic processing were included. Expression patterns of selected five genes were validated by quantitative real time PCR. The results of this analysis support that the eutopic endometrium from patients with advanced-stage endometriosis has distinct gene expression profile from eutopic endometrium of control without endometriosis.
Highlights
Endometriosis is defined as the presence of endometrial tissue outside the uterus, and this condition affects 6% - 10% of women of reproductive age
Five endometriosis patients who were confirmed by laparoscopy or laparotomy as being in a moderate or severe stage according to the guidelines of American Society for Reproductive Medicine (1997) and five patients with no endometriosis detected in surgical findings and pathohistological examination were selected; their endometriums were qualified as being in the late secretory phase
In order to examine gene expression changes in relation to endometriosis, this study obtained the endometrial tissues of five endometriosis patients and five other patients with no endometriosis whose endometriums were in the late secretory phase
Summary
Endometriosis is defined as the presence of endometrial tissue outside the uterus, and this condition affects 6% - 10% of women of reproductive age. The etiology of endometriosis remains elusive, retrograde menstruation into the peritoneal cavity during menstruation is widely accepted as a major contributing factor in the pathogenesis of this disease. Backflow of menstrual materials through the fallopian tubes into the pelvic cavity is a common phenomenon that occurs in up to 90% of menstruating women with patent fallopian tubes [3]. It has yet to be determined why endometriosis affects only a certain group of women. This finding suggests that there may be immunological and genetic factors that determine a woman’s susceptibility to endometriosis
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