Abstract

DNA methylation is known as an important epigenetic change in plants and vertebrates genome. In this process, the methyl group transferred by DNA methyl transferase enzymes to cytosine at carbon residue 5 often in the CpG dinucleotide context. DNA methylation plays an important role in the natural development of the organism, genome stability maintenance and processes such as genomic imprinting and chromosome X inactivation in mammals. In addition, changes in DNA methylation pattern have seen in many diseases, including cancer. Analysis of DNA methylation has been useful for rapid disease diagnosis and progression. In recent decades, a revolution has taken place in the methods of DNA methylation analysis, and it is possible to study the pattern of gene methylation at a widespread, short and high resolution level. These methods can be divided into three general categories: (1) cut-based methods by methylation-sensitive enzymes; (2) sodium bisulfide based methods; (3) antibody based methods. Since the existence of different methods makes it difficult to select the appropriate approach, in this review, a number of common methods for examining the methylation pattern with the advantages and disadvantages will be discussed.

Highlights

  • DNA methylation known as the first epigenetic modifications reported in mammalian cells [1]

  • Two most well characterized chromatin remodeling processes including: 1) DNA methylation 2) histone post-translational modification [5]

  • Epigenetic alteration triggered in response to environmental exposures to modulate the cellular function [6,7] which included adding a methyl group DNA methyltransferase covalently to 5’-carbon of cytosine mostly in CpG dinucleotide [8,9]

Read more

Summary

Introduction

DNA methylation known as the first epigenetic modifications reported in mammalian cells [1]. This method is a high throughput method to the analysis of genomic DNA methylation based on the use of combined DNA cleavage by methylation-sensitive restriction enzymes and polymerase extension assay by pyrosequencing. The first step in this process is DNA digestion with a methylationsensitive enzyme such as HpaII, and all CCGG sites cleave with a non-methylation-sensitive enzyme such as MspI.

Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call