DNA methylation in silkworm genome may provide insights into epigenetic regulation of response to Bombyx mori cypovirus infection

Ping Wu,Wencai Jie,Enoch Annan,Chenxiang Hou,Qi Shang,Xijie Guo,Xiaoxu Jiang,Tao Chen

doi:10.1038/s41598-017-16357-7

Abstract

DNA methylation is an important epigenetic modification that regulates a wide range of biological processes including immune response. However, information on the epigenetics-mediated immune mechanisms in insects is limited. Therefore, in this study, we examined transcriptomes and DNA methylomes in the fat body and midgut tissues of silkworm, Bombyx mori with or without B. mori cytoplasmic polyhedrosis virus (BmCPV) infection. The transcriptional profile and the genomic DNA methylation patterns in the midgut and fat body were tissue-specific and dynamically altered after BmCPV challenge. KEGG pathway analysis revealed that differentially methylated genes (DMGs) could be involved in pathways of RNA transport, RNA degradation, nucleotide excision repair, DNA replication, etc. 27 genes were shown to have both differential expression and differential methylation in the midgut and fat body of infected larvae, respectively, indicating that the BmCPV infection-induced expression changes of these genes could be mediated by variations in DNA methylation. BS-PCR validated the hypomethylation of G2/M phase-specific E3 ubiquitin-protein ligase-like gene in the BmCPV infected midgut. These results demonstrated that epigenetic regulation may play roles in host-virus interaction in silkworm and would be potential value for further studies on mechanism of BmCPV epithelial-specific infection and epigenetic regulation in the silkworm.

Highlights

Pathogens infection, silkworm strain, and climatic condition have drastic effects on the yield and quality of the silk produced
The correlation between gene expression and DNA methylation patterns was analyzed. This is the first report on exploring the epigenetic regulation mechanisms on the immune response of B. mori and our findings provide insight into the transcriptional and epigenetic mechanisms underlying the interactive responses between silkworm and pathogens
Quantitative real-time PCR results revealed that the expression of B. mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene in fat bodies of infected silkworms was significantly lower than in midguts, suggesting that fat body was not infected by BmCPV (Supplementary Fig. S1)

Summary

Introduction

Silkworm strain, and climatic condition have drastic effects on the yield and quality of the silk produced. In this study, we profiled DNA methylomes and transcriptomes in the fat body and midgut tissues of B. mori with or without BmCPV infection using whole-genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-Seq) technologies. We detected 737 differentially expressed genes, among which, the transcript level of 162 genes was increased while 575 genes was decreased in response to BmCPV infection (Supplementary Table S3).

Results

Conclusion