Abstract
It is well established that DNA cytosine methylation epialleles (CG vs 5MeCG) alter the expression of obesity related genes. The current study examined differences in 5MeCG in CD16+ cells isolated from blood using antibody‐bound magnetic beads in normal weight (BMI 18.5‐24.9 kg/m2; n = 12) and obese (BMI > 30 kg/m2; n = 6) women (18‐35y). 5MeCG was measured across > 485,000 CG sites using the HumanMethylation450 BeadChip (Illumina©). 16,969 CG sites were differentially methylated in obese as compared to normal weight women (p < 0.05). 65.4% of these sites had decreased methylation. Six of these CG’s remained associated (p < 10‐7) after adjustment for multiple testing (False Discovery Rate < 0.05), including sites in CRYGB, CDH5, and VASH1. Our results have potential implications in obesity and CDH5 has been shown previously to have differential methylation in peripheral blood leukocytes in obese (Wang et al. doi: 10.1186/1741‐7015‐8‐87). Continued analysis will further examine these methylation differences.Grant Funding Source: Supported by a grant from the UGA Obesity Initiative and the GRA Eminent Scholar Endowment (CAB)
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