Abstract
Objective To explore the expression of human telomerase reverse transcriptase (hTERT) in intrahepatic cholangiocarcinoma cell line RBE transfected with hepatitis C virus core protein (HCVc) plasmid,and involvement of DNA methy ltransferase 1 (DNMT1) in the up-regulation of hTERT induced by HCVc.Methods Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to analyze the expression of hTERT and DNMT1 after the transfection of HCVc plasmid into RBE cells.5-Aza-2'-deoxycytidin (AZA) was used to inhibit DNMT1.Results hTERT expression level was higher in RBE cells after HCVc transfection and DNMT1 expression was up-regulated simultaneously.The mRNA and protein levels of hTERT were up-regulated by 3.457 ± 0.081 and 1.684 ± 0.020 respectively (P ＜ 0.05).And the expression levels of DNMT1 were up-regulated by 1.826 ± 0.060 and 2.513 ± 0.119 respectively (P＜ 0.05).After treatment with AZA,the mRNA and protein levels of hTERT were down-regulated by 2.755 ± 0.098 and 1.491 ± 0.045 respectively (P ＜ 0.05).Conclusion DNMT1 may take part in the up-regulation of hTERT induced by HCVc. Key words: Cholangiocarcinoma; Hepatitis C virus core protein; DNA methyltransferase; Telomerase
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