DNA metabarcoding illustrates biological pollution threats of Red Sea - Dead Sea water conveyance to Dead Sea biodiversity

Abstract

The Dead Sea has a hypersaline environment where only extremophile species like Archaea, Bacteria, and fungi can survive. The Red Sea-Dead Sea Water Conveyance (RDSC) is constructing a pipeline of 180 km to import water from the Red Sea in the shrinking Dead Sea. Both seas exhibit highly different hydrographic features that determine their biodiversity. Using environmental DNA (eDNA) metabarcoding and amplifying a fragment of the cytochrome oxidase subunit I gene (COI) from water samples, we compared the communities of the Red Sea and the Dead Sea to understand the potential impact of the water conveyance project on biodiversity, following by an identification of potential biopollutants able to enter the Dead Sea for their small size. The results suggest a high likelihood of acquiring harmful algae into the Dead Sea. This study alerts about the real risk of losing the unique Dead Sea biota when the conveyance is actually undertaken.