DNA Isolation of Chicken Feathers from the Base of the Young Feathers, the Base of the Old Feathers, and the Tip of the Feathers

Alfi Sophian

doi:10.22236/j.bes/526211

Abstract

Background: The goal was to provide information on the use of DNA templates in chicken samples so that the molecular research sampling process may employ feathers instead of hurting the test animals. Method: The sample used consisted of 10 Bangkok chickens which were sampled for young feathers and old feathers and the tips of the feathers. Quantitative techniques by comparing the results of DNA isolation which were analyzed using a nano photometer and then confirmed using real-time PCR with the SYBR green method. Result: The analysis of purity and concentration showed that at the base of young chicken feathers, the average value of purity was at 1,790, with an average value of the concentration of 4,210. At the base of the old feather, the average value of purity was 0.638, with an average concentration value that was not detected. Likewise, at the tip of the feather, the average purity value is 0.894 and the concentration value is not detected. Confirmation tests performed on all samples using the real-time PCR melt curve method showed that all samples were detected with a Tm value of 78.5 for young feathers, 78.5 for old feathers, 79.0 for positive controls and 78.7 for positive controls, while negative controls were not detected. Conclusion: DNA isolation can be carried out at the base of the young feathers, the base of the old feathers and the tips of the feathers.

Highlights

Analisis Hasil Isolasi DNA dari Bulu Ayam yang Dicuplik dari Pangkal Bulu Muda, Pangkal Bulu Tua dan Ujung Bulu
DNA isolation through a red chloroform extraction process, the sample will go through three main processes, namely lysis, separation of DNA from protein/cellulose and purification
The selection of chicken samples is due to the large number of studies using chickens with limited reference information regarding sampling as the object of research

Summary

Introduction

Analisis Hasil Isolasi DNA dari Bulu Ayam yang Dicuplik dari Pangkal Bulu Muda, Pangkal Bulu Tua dan Ujung Bulu. DNA isolation through a red chloroform extraction process, the sample will go through three main processes, namely lysis (destruction of cell walls), separation of DNA from protein/cellulose and purification Each of these stages plays an essential role in the success of the DNA isolation process to get good results, namely. In a DNA analysis using animal samples, the most important thing to note is the ethical issue of the object of research. This problem is fundamental to pay attention to because it concerns how the test animals used as the object of research do not experience pain or pain when the sample is taken. The novelty aspect of this research lies in the initial idea of selecting a sample that will be the reference source for similar research

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Conclusion