Abstract
Abstract. Title ofthe paper: Investigations of population genetic parameters using multilocus DNA fingerprinting in 4 goat breeds in Germany Conventional fmgerprint banding pattems were produced by Southern blotting and digoxigenm labeled oligonucleotid probes (GTG)5 and (GT)g. Digitised images of the blots were analysed with the RFLPscan Computer program. These probes produced more bands (20–39 distinct informative bands within each group) in these goat breeds than in sheep breeds. Typically, in individual goats, an average of 8–11 bands were detected in the range of 4.3–23 kb. Analysis ofthe Polymorphie banding pattems showed that 4.5 to 8.3 genetic loci were represented by these probes. The analysis of pooled DNA samples (10–28 animals per lane) overestimated similarity values (S) between different breeds. Average similarity values within groups (based on RFLPs of individuals) ränge from 0.42–0.61, while similarity values between different groups ränge from 0.36–0.47. The results indicated that native Thüringer Wald Ziege are different from the Toggenburger goats.The Heterozygosity index (H) for each breed calculated from (GTG)5 fingerprints (H = 0.42–0.62) were in good agreement (negative correlated) with band sharing valve. These H values are lower than those obtained in a similar study of 19 sheep breeds in which the ränge was (0.68–0.82). The goat breed with the highest H value was the Bunte Deutsche Edelziege (H = 0,62). Ranking the breeds by heterozygosity index and by inversely band sharing index gives: BDE > German TOGO > WDE, TWZ > Swiss TOGO. The results are discussed with respect to breeding programs and the influence of small population size.
Highlights
Mittlerer Ähnlichkeitsindex und genetische Distanz zwischen den Ziegenrassen anhand der RFLPs mit der (GTG)s-Sonde von Einzeltieranalysen (Average similarity and genetic distance between 4 breeds of goats calculated from DNA fingerprints of single animals) mittlerer S-Index 2
Mittlere BS-Werte und mittlere Heterozygotie in der DNA bei den 4 Ziegenrassen aus Einzeltieranalysen mit multi-locus-Fingerprints (Average band frequences, band sharing values and heterozygosity calculated from individual DNA fingerprints in goat breeds)
Nach HERMANS et al (1993) haben sich die in den Tabellen aufgeführten Parameter als geeignete Indikatoren für die genetische Diversität bei Schafrassen erwiesen, während andere einschlägige Arbeiten dafür auch die mittlere Heterozygotie nach STEPHENS et al (1992) genutzt haben (GILBERT et al, 1991; GATEI et al, 1991; BAKER et al, 1993; MATHUR et al, 1994; ROGSTAD and PELIKAN, 1996)
Summary
Title ofthe paper: Investigations of population genetic parameters using multilocus DNA fingerprinting in 4 goat breeds in Germany Conventional fmgerprint banding pattems were produced by Southern blotting and digoxigenm labeled oligonucleotid probes (GTG) and (GT)». The results indicated that native Thüringer Wald Ziege are different from the Toggenburger goats.The Heterozygosity index (H) for each breed calculated from (GTG) fingerprints (H = 0.42-0.62) were in good agreement (negative correlated) with band sharing valve These H values are lower than those obtained in a similar study of 19 sheep breeds in which the ränge was (0.68-0.82). RFLPs-Analysen an Ziegenrassen zur Charakterisierung von Populationen sollten wie bei Wildtieren oder anderen Nutztieren (JEFFREYS und MORTON, 1987; GEORGES et al, 1988; GILBERT et al, 1991; STEPHENS et al, 1992; BAKER et al, 1993; TSURUGA et al, 1994) von Interesse sein, weil sich nach einschlägiger Literamr beispielweise hier enge Zuchtprogramme bei den ausgeprägten Leistungsrassen oder kleine Bestandszahlen auf die genetische Varianz bei gefährdeten Rassen negativ auswirken können und durch verschiedene Parameter zu erfassen sind.
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