Abstract
AbstractA collection of 11 coloured cotton Gossypium hirsutum genotypes and four white linted genotypes of different origin were evaluated by randomly amplified polymorphic DNA (RAPD) analysis. These 15 cotton genotypes were evaluated using 32 different 10‐mer primers of arbitrary sequences. All 32 primers were polymorphic ‐ in total, 287 amplified fragments were observed in these patterns. Out of the 287 fragments, 219 were polymorphic accounting for 76.31% of the total number of fragments. Similarity indices were calculated using the Dice coefficient and a dendrogram showing relationships between genotypes was obtained by Unweighted Pair Group Method of Arithmetic Average (UPGMA) cluster analysis. Cluster analysis showed clear‐cut separation of the coloured and white linted genotypes and thus formed three clusters (I, II and III). Among the coloured linted genotypes, all except ‘Parbhani American’ and ‘Lousiana brown’ clustered together. Cluster II contained white linted genotypes orginating from the same breeding station. The results indicate that RAPDs may constitute a relatively simple and efficient method for analysing genetic variation in coloured and white linted G. hirsutum collections.
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