DNA Fingerprinting of Cold Stress Tolerant and Susceptible Chickpea Genotypes with RAPD Markers

Abstract

Sixteen cold stress tolerant and two cold stress susceptible chickpea cultivars were investigated for their genetic diversity and relationship using 15 RAPD primers. The genotypes showed a considerable diversity with 50–100% percent polymorphism. Polymorphic information content and resolving power varied from 0–0.71 and 0.22–2.76, respectively. The dendrogram based on Jaccard's similarity coefficient separated 18 chickpea genotypes into 5 main clusters but ICCV92944 remained alone in one cluster. The two cold stress susceptible genotypes PBG-1 and GPF-2 clustered together and showed divergence from other cultivars. ICCV88503 occupied a unique position in the dendrogam as it remaind unclustered and revealed most diverse genetic nature from rest of 17 cultivars of chickpea. Genotypes IPC 98–51 and GC28028 showed maximum similarity.