Abstract
Genomic DNA was prepared from a total of 16 different isolates of the rape seed pathogen Leptosphaeria maculans , the teleomorph of Phoma lingam . These DNA preparations were digested with the restriction endonucleases Bam H I, Eco R I, Hpa II, Mbo I, Msp I and Sau3 A I, separated on agarose gels and analysed by hybridization with the radioactively labelled synthetic oligonucleotides (GTG) 5 , (GACA) 4 or (CT) 8 or with labelled DNA of phage M13. These probes reveal a high degree of DNA polymorphism within the species. Although all investigated isolates can clearly and unequivocally be distinguished from each other, aggressive and nonaggressive strains appear as consistent groups with high degrees of similarity. The fingerprinting technique is thus an appropriate tool for molecular diagnosis of this important plant pathogen. In general, oligonucleotide fingerprinting may be regarded as a convenient technique to identify individual fungal strains with a reliability similar to that of mammalian systems.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
