DNA Fingerprinting Construction and Genetic Diversity Analysis for Azuki Bean (Vigna angularis) and Mung Bean (Vigna radiata L.) Germplasm Resources

Abstract

Background: As a coarse grain variety, azuki beans and mung beans were widely grow in Asia. Due to the lack of management in the long-term cultivation process, leading to the mixed phenomenon among azuki bean and mung bean varieties, which seriously affects the cultivation and breeding of azuki beans and mung beans. The current study aims to construct the genetic map using SSR markers for the genetic classification of beans. Methods: In 2019, 46 test materials were raised to extract the young leaves. The DNA fingerprinting of 46 cultivated azuki bean and mung bean resources were constructed using 38 pairs of SSR primers and their genetic diversity were analyzed. Azuki bean and mung bean varieties are divided by hierarchical clustering analysis. Result: Totally 18 primers have shown polymorphism among the 38 primers and 188 polymorphic bands were amplified with average PIC of value 0.8302. According to the K mean cluster analysis method, 23 azuki beans and 23 mung beans resources were grouped into three categories. According to the hierarchical cluster analysis method, azuki beans were divided into four categories and mung beans were divided into six categories. The 46 azuki bean germplasm resources could be distinguished by screening nine pairs of digital fingerprint constructed by core primers.