Abstract
DNA binds Fe(II) in a manner such that OH formation from H 2O 2 is enhanced as compared to the amount formed in the absence of DNA. Hydroxyl free radical formation was assayed using the spin-trap DMPO (5,5-dimethyl pyrroline-1-oxide). The maximum ȮH formation occurs at an added Fe(II) per nucleotide ratio of about 1 70 . The ȮH formed from H 2O 2 by the DNA-Fe(II) complex attacked DNA producing aldehydes as determined by the thiobarbituric acid method. These data implicate DNA strand breaks induced by ȮH attack.
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