Abstract
Tuberculosis (TB) remains as a major public health issue in developing countries. Accurate detection is essential for the proper management of patients with active disease. Here, we present a simple DNAzol-LAMP (loop-mediated isothermal amplification) procedure for the detection of Mycobacterium tuberculosis in sputum specimens. Twenty smear-positive sputum samples were analyzed as follows: (i) Genetic material was extracted by a standard DNAzol protocol, and (ii) mycobacterial DNA was detected by a typical TB-specific loop-mediated isothermal amplification method. Results and diagnostic test performance attests to the suitability of the proposed procedure.
Highlights
Tuberculosis (TB) continues among the major threats to public health
The study was conducted in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee of the General Hospital of Tijuana (Project identification code 10/05/2016)
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Summary
Tuberculosis (TB) continues among the major threats to public health. In 2015, the WHO estimated 10.4 million new cases [2], suggesting that both late detection and misdiagnosis sustain the disease prevalence [3]. Smear microscopy, and bacterial culture, are the foremost means for the detection of Mycobacterium tuberculosis, the leading causative agent of TB in humans [4]. But has low sensitivity, while bacterial culture (the gold standard) is sensitive, but it takes up to six weeks to provide a reliable result. Despite their intrinsic disadvantages, to date, these methods are applied for routine mycobacteriology [5,6]
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