Abstract
A specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses.
Highlights
Sea anemones (Anthozoa: Actiniaria) are simple askeletal polyp animals, and a very diverse, ecologically important group of organisms (Schick, 1991)
Total genomic DNA was extracted from pieces of 100% ethanol-preserved sea anemones
DNA extracted from sea anemone samples was used as a template in polymerase chain reaction (PCR) amplifications with the single primer amplification reaction technique (SPAR) technique (Gupta et al, 1994)
Summary
Sea anemones (Anthozoa: Actiniaria) are simple askeletal polyp animals, and a very diverse, ecologically important group of organisms (Schick, 1991). There are few techniques available for the extraction of DNA from sea anemone species (e.g., Wolstenholme, 1992; Pont-Kingdon et al, 1994; Finnerty and Martindale, 1997; Fautin and Smith, 1997) and these studies followed standard protocols previously described for other metazoan organisms (e.g. those of Wolstenholme and Fauron, 1976; Shure et al, 1983; Winnepenninckx et al, 1993; Folmer et al, 1994). We have had success in obtaining good DNA templates using an optimization of the protocol described by Chen et al (1995) to extract and subsequently amplify DNA from sea anemone specimens.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
