DNA Extraction from Pulp Tissue for its Application in PCR. A Study to Extract DNA with High Purity as Quantified by Gel Electrophoresis

Abstract

ABSTRACT The isolation of human genomic DNA is an important step involved in genetic identity analysis. Also getting optimal quantity and quality of DNA is important as this can alter the results. This consideration is especially important in the field of forensic analysis and genetic identity, where DNA is extracted from extremely small amounts of starting material often recovered from suboptimal storage conditions. Odontological examinations have always been a critical determinant in the search of human identity. Teeth form a natural barrier to exogenous DNA contamination. DNA extraction from dental tissues both hard tissues and dental pulp, has been reported the use of dental pulp, as a source of DNA offers a new paradigm in the process of DNA extraction and its use in forensic analysis.11 The following procedures elucidate a novel method of extraction of DNA using dental pulp, which offers a cost-effective and attractive procedure, which can be replicated and applied in DNA amplification (PCR).