Abstract
Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10−13 M with a wide detection range from 1.0 × 10−6 to 1.0 × 10−12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis.
Highlights
Mycobacterium tuberculosis (MTB) is an infectious bacterium responsible for tuberculosis (TB)pulmonary disease [1]
The results show that the electroactive surface area of screen-printed carbon electrode (SPCE)/mercaptopropionic acid (MPA)-Fe3 O4 /Nanocellulose crystalline (NCC)/cetyltrimethylammonium bromide (CTAB) was increased by approximately 54.5% compared to the bare
The present study demonstrated a sensitive electrochemical DNA biosensor platform based on a new composite nanomaterial
Summary
Mycobacterium tuberculosis (MTB) is an infectious bacterium responsible for tuberculosis (TB)pulmonary disease [1]. TB affects people of both sexes of all ages. The largest group involves men rather than women (aged ≥ 15 years), who accounted for 57% of all the cases. Among all TB cases, 8.6% were people living with HIV [2,3]. Various efforts have been carried out to improve TB diagnosis to reduce the number of deaths as set by the World Health Organization (80% reduction in TB incidence rate per year). These only can be achieved if the high-risk people are diagnosed correctly. The most effective treatment regimen should be started as early as possible
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