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Abstract
The mitochondrial DNA (mtDNA) of a grande strain and three petite mutant strains of yeast, Saccharomyces cerevisiae, has been analyzed by DNA-DNA filter hybridization and CsCl isopyknic density gradient centrifugation. All strains genetically transmitted either chloramphenicol or erythromycin resistance or both to sensitive nonpetite strains. The grande mtDNA had a buoyant density of 1.683 g/cm3, and the three petite mtDNAs had buoyant densities of 1.683, 1.682, and 1.689 g/cm3, respectively. The hybridization between 3H-labelled grande mtDNA and each of the three petite mtDNA filters was 42%, 11%, and 4% of isohybridization levels. Thermal denaturation of the hybrids indicated very specific hybridization. Homology between petite mtDNA was greatest in petites retaining common genetic markers for antibiotic resistance, and was least when different markers were retained. The results are consistent with deletion of different segments of grande mtDNA in the petite mutant.
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