Abstract
Fixed mouse kidney epithelial cells have been examined for their capacity to synthesize RNA with their own RNA polymerases when supplied with ribonucleoside triphosphates. The endogenous polymerase activity of chromatin in fixed cells is clearly related to changes in the size and protein content of the nucleus. Cells with small nuclei which do not incorporate 3H-uridine in vivo show very little RNA polymerase activity at the ionic strength of the standard assay procedure. This activity can be enhanced by increasing the ionic strength of the assay medium. Changes in RNA polymerase activity also appear to be related to changes in the ability of chromatin to bind acridine orange (AO).
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