DNA-damaging agents induce the 72-kD heat shock protein in SV40 transformed normal human fibroblasts.

Abstract

In order to elucidate the involvement of DNA damage in the induction of heat shock proteins (stress proteins), we examined the induction of 72-kD heat shock protein (HSP72) in an SV40-transformed human fibroblast cell line (WI38VA13) which was exposed to various DNA-damaging agents, including 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3 -nitro-methylmethane sulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, and 4-nitroquinoline-N-oxide. Induction of HSP72 was detected by the indirect immunofluorescence method using a monoclonal antibody. All the DNA-damaging agents used in this study induced HSP72 on human fibroblasts. This result indicates that DNA damage is one trigger for the induction of HSP72.