DNA damage protective effect of honey-sweetened cashew apple nectar in Drosophila melanogaster.

Robson Alves Da Silva,Bianca Regina Ribas De Abreu,Lucas Pinheiro Dias,Mauricio Lehmann,Maiane Papke Costa,Rafael Rodrigues Dihl,Kênya Silva Cunha

doi:10.1590/1678-4685-gmb-2015-0129

Robson Alves Da Silva, Bianca Regina Ribas De Abreu + Show 5 more

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https://doi.org/10.1590/1678-4685-gmb-2015-0129

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Abstract

Fruits and derivatives, such as juices, are complex mixtures of chemicals, some of which may have mutagenic and/or carcinogenic potential, while others may have antimutagenic and/or anticancer activities. The modulating effects of honey-sweetened cashew apple nectar (HSCAN), on somatic mutation and recombination induced by ethyl methanesulfonate (EMS) and mitomycin C (MMC) were evaluated with the wing spot test in Drosophila melanogaster using co- and post-treatment protocols. Additionally, the antimutagenic activity of two HSCAN components, cashew apple pulp and honey, in MMC-induced DNA damage was also investigated. HSCAN reduced the mutagenic activity of both EMS and MMC in the co-treatment protocol, but had a co-mutagenic effect when post-administered. Similar results were also observed with honey on MMC mutagenic activity. Cashew apple pulp was effective in exerting protective or enhancing effects on the MMC mutagenicity, depending on the administration protocol and concentration used. Overall, these results indicate that HSCAN, cashew apple and honey seem capable of modulating not only the events that precede the induced DNA damages, but also the Drosophila DNA repair processes involved in the correction of EMS and MMC-induced damages.

Highlights

The ingestion of dietary components that decrease DNA damage accumulation may be an effective strategy for either the modulation or the inhibition of the carcinogenic process (Pingitore et al, 2015)
Because of the weak expression of the flr3 marker in small clones and its lethality in large clones of mutant cells (Graf, 1995), only the mwh clones were used to calculate the clone formation frequencies per 105 cells. These values were employed to estimate the contribution of recombination and mutation to the incidence of total mutant spots per fly in trans-heterozygous flies. The analysis of both mwh/flr3 and mwh/TM3 genotypes allowed to evaluate the effect of co- and post-treatment with honey-sweetened cashew apple nectar (HSCAN) on the genotoxicity of ethyl methanesulfonate (EMS) (Table 1), and the effect of the treatment with HSCAN, cashew apple pulp and honey on the genotoxicity of mitomycin C (MMC) (Table 2)
The chemopreventive activity of HSCAN, cashew apple pulp and honey on DNA damage induced by EMS and MMC was investigated with the Drosophila wing somatic mutation and recombination test (SMART), using coand post-treatment protocols

Summary

Introduction

The ingestion of dietary components that decrease DNA damage accumulation may be an effective strategy for either the modulation or the inhibition of the carcinogenic process (Pingitore et al, 2015). It is essential to assess safety and efficacy of candidate chemopreventive agents in preclinical models and in humans, and to understand their mechanisms of action (Brown et al, 2014; Liang and Kitts, 2014; Sloczynska et al, 2014). Fruits and their derivatives, such as juices, are complex mixtures of chemicals. The protective activity of two components of HSCAN, cashew apple pulp and honey, was evaluated This bioassay allows the simultaneous detection and quantification of mitotic recombinations versus gene and chromosomal mutations (de Andrade et al, 2004)

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