Abstract
The prooxidant effect of resveratrol (3,5,4′-trihydroxy- trans-stibene) and its synthetic analogues (ArOH), that is, 3,4,4′-trihydroxy- trans-stibene (3,4,4′-THS), 3,4,5-trihydroxy- trans-stibene (3,4,5-THS), 3,4-dihydroxy- trans-stibene (3,4-DHS), 4,4′-dihydroxy- trans-stibene (4,4′-DHS), 2,4-dihydroxy- trans-stilbene (2,4-DHS), 3,5-dihydroxy- trans-stilbene (3,5-DHS) and 3,5,4′-trimethoxy- trans-stibene (3,5,4′-TMS), on supercoiled pBR322 plasmid DNA strand breakage and calf thymus DNA damage in the presence of Cu (II) ions has been studied. It was found that the compounds bearing ortho-dihydroxyl groups (3,4-DHS, 3,4,4′-THS, and 3,4,5-THS) or bearing 4-hydroxyl groups (2,4-DHS, 4,4′-DHS, and resveratrol) exhibit remarkably higher activity in the DNA damage than the ones bearing no such functionalities. Kinetic analysis by UV-visible spectra demonstrates that the formation of ArOH-Cu (II) complexes, the stabilization of oxidative intermediate derived from ArOH and Cu (II)/Cu (I) redox cycles, might be responsible for the DNA damage. This study also reveals a good correlation between antioxidant and prooxidant activity, as well as cytotoxicity against human leukemia (HL-60 and Jurkat) cell lines. The mechanisms and implications of these observations are discussed.
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