DNA damage in foundry workers using non-invasive micronucleus cytome assay.

Abstract

Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three exposed foundry workers as a exposure groups and 33 non-exposed individuals as a control groups participated in this study. Buccal micronucleus cytome (BMCyt assay) assay was used to assess DNA damage. Results showed that foundry workers were under exposure to hazardous chemical and physical agents such as metal fumes and noise. The percentage of micronucleus (MN) cells in exposure group (0.59±0.93 %) were statistically higher than control group (0.23±0.23 %) (P<0.05) %). Also, the percentage of nuclear bud cells and binucleated cells in exposure group were statistically higher than control group (P<0.05). The percentage of differentiated normal cells were significantly higher in the control group compared to the exposed group (P<0.05). Foundry workers are at risk of DNA damage; therefore, prevention measures need to be implemented to reduce exposure to air pollutants in foundry workplaces.