DNA damage by oxidized fatty acids detected by DNA/SPE biosensor

Ľudmila Sirotová,Marcela Matulová

doi:10.36547/nbc.1307

Abstract

Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.

Highlights

The damage of DNA and its consequences have become the closely observed topics in the last years (MARNETT, 2000; MARNETT et al, 2003)
This could be due to the oxidative products already present in the samples, what can be confirmed by PV and thiobarbituric acid reactive substances (TBARS) values determined for the respective fatty acids (Fig. 2 and 3)
The DNA/SPE biosensor detected the bigger extent of the DNA damage in the case of the reaction mixture of oxidized linoleic acid compared to oxidized oleic acid

Summary

Introduction

The damage of DNA and its consequences have become the closely observed topics in the last years (MARNETT, 2000; MARNETT et al, 2003). Some of the studies suggest that the oxidation of fatty acids and the consequent DNA damage play an important role in such processes (MARNETT, 2002; KANNER, 2007). The first products of polyunsaturated fatty acid oxidation are relatively short-lived lipid hydroperoxides. They are reduced to unreactive fatty acid alcohols or react with metals to aldehydes, ketones, alcohols, short fatty acids, esters, hydrocarbons, furans, and lactones, named as secondary products (BURCHAM, 1998). The phosphodiester backbone can be damaged, leaving abnormal ends. Such alterations to DNA have been shown to disrupt transcription, translation, DNA replication and lead to mutations, cell senescence or death (TERMINI, 2000; BLAIR, 2001)

Methods

Results

Conclusion