Abstract
Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet assay was used to measure DNA strand breakage, 8-oxoguanine levels and apoptotic and necrotic nuclei in after admission (day 0) and on days 3, 5, 7 and 15. TAOS was determined by colorimetric method. Results: Trauma patients had high DNA damage at admission (p < 0.01), that further increased with maximum value on day 5 (p < 0.001). On day 15 the degree of DNA damage remained significantly elevated (p < 0.01). No significant difference in the 8-oxoguanine levels at all days examined was found. Patients had a high percentage of apoptotic and necrotic comets at admission, with maximum values on days 3 and 5. A significantly lower TAOS was observed in patients on admission and days 3, 5, 7 and 15 (p < 0.001 in all cases). A decreasing of TAOS on days 7 and 15 compared to that on admission (p < 0.05) was observed. Conclusions: Blood cells from severe trauma patients’ display increased DNA damage associated with apoptosis and necrosis. Reduced plasma TAOS and a tendency to increase of 8-oxoguanine in DNA was observed.
Highlights
Severe multiple trauma remains as one of the major problems of contemporary medicine and society throughout the world
The Single Cell Gel Electrophoresis assay (SCGE), or comet assay are sensitive method for the evaluation of DNA damage from individual cells based on the migration of denatured DNA through an electrophoretic field [14]
The DNA comet assay is sensitive method for the evaluation of DNA damage from individual cells based on the migration of denatured DNA through an electrophoretic field [14]
Summary
Severe multiple trauma remains as one of the major problems of contemporary medicine and society throughout the world. Experimental evidences suggest that MOF in the event of trauma might be relates to stress-induced cell death by apoptosis, but its exact mechanisms is not fully understood [8,9,10,11,12]. The Single Cell Gel Electrophoresis assay (SCGE), or comet assay are sensitive method for the evaluation of DNA damage from individual cells based on the migration of denatured DNA through an electrophoretic field [14]. The aim of this pilot study was an assessment DNA strand breakage, 8-oxoguanine levels and cellular death of white blood cells and their possible connection with total antioxidant status in patients with severe multiple trauma
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