Abstract

Abstract In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.

Highlights

  • The fig (Ficus carica L., 2n=2x=26, diploid, CONDIT, 1928) and black mulberry (Morus nigra L., 2n=22x=308, decosaploid, AGAEV; FEDOROVA, 1970; BASAVAIAH et al, 1990) belong to the Eusyce section and Moreae tribe, respectively, of the Urticales order Moraceae family which comprises 37 genera and approximately 1,100 species

  • Feulgen densitometry (FD) and flow cytometry (FC) are the two mostly used methods in DNA content estimation. The latter has been used to estimate the nuclear DNA content and genome size of an unknown sample comparing with a reference standard (GALBRAITH et al, 1983; DOLEŽEL et al, 1998; DOLEŽEL; GREILHUBER, 2010; TİRYAKİ; TUNA, 2012)

  • Diploid barley (Hordeum vulgare L., 2n=2x=14, 2C=10.65-10.68 pg, 1C=5,022-5,218 Mbp, TİRYAKİ; TUNA, 2012; TUNA et al, 2001; BENNETT; LEITCH, 2011) and diploid common vetch (Vicia sativa L., 2n=2x=12, 2C=3.69-3.79 pg, 1C=1,808-1,857 Mbp, KAHLAOUI et al, 2009) are among the internal reference standards used in FC

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Summary

Introduction

The fig (Ficus carica L., 2n=2x=26, diploid, CONDIT, 1928) and black mulberry (Morus nigra L., 2n=22x=308, decosaploid, AGAEV; FEDOROVA, 1970; BASAVAIAH et al, 1990) belong to the Eusyce section and Moreae tribe, respectively, of the Urticales order Moraceae family which comprises 37 genera and approximately 1,100 species. These two fruit species have of economic and social importance in agriculture, and health benefits. The 2C DNA contents per nucleus for diploid fig were estimated as 1.41±0.01 pg using FD (OHRI; KHOSHOO, 1987) and as 0.73±0.03 pg using propidium iodide (PI) (LOUREIRO et al, 2007b). The 2C DNA content of F. bengalensis was 1.45±0.01 pg using FD (OHRI; KUMAR, 1986) These were the four records for Ficus spp. To acquire some knowledge on the DNA content on cultivated fig and black mulberry will help to facilitate genomics studies. The objective of this study was to determine 2C DNA content of fig and black mulberry using DAPI in flow cytometry. This is the first report on determining of 2C DNA content of fig and black mulberry using DAPI

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