Abstract
Porphyrins and their metal derivatives are strong DNA binders with association constants of around 106 M-1. Some of these compounds have been used for radiation sensitization therapy of cancer and are targeted to interact with cellular DNA. Chlorophyllin (CHLN), a food-grade derivative of chlorophyll (CHL), the ubiquitous green plant pigment widely consumed by humans, is a potent inhibitor of experimental carcinogenesis. The aim of this study was to examine the interaction of calf-thymus DNA with chlorophyllin in aqueous solution at physiological pH, with CHLN/DNA(phosphate) molar ratios (r) of 1/80, 1/40, 1/20, 1/10 1/4, and 1/2. Fourier transform infrared (FTIR) difference spectroscopy was used to determine the CHLN binding mode, binding constant, sequence selectivity, DNA secondary structure, and structural variations of the DNA−CHLN complexes in aqueous solution. Spectroscopic evidence showed that at low pigment concentration (r = 1/80), CHLN binds DNA via intercalative mode into the G−C and A−T-rich ...
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