DNA Biosensor Based on Double-Layer Discharge for the Detection of HPV Type 16.

Espinosa Espinosa,Ayala Ayala,Galván Galván,Quiñones Quiñones,Durón Durón

doi:10.3390/s19183956

Espinosa Espinosa, Ayala Ayala + Show 3 more

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https://doi.org/10.3390/s19183956

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Abstract

DNA electrochemical biosensors represent a feasible alternative for the diagnosis of different pathologies. In this work, the development of an electrochemical method for Human Papillomavirus-16 (HPV-16) sensing is reported based on potential relaxation measurements related to the discharge of a complex double layer of a DNA-modified gold electrode. The method used allows us to propose an equivalent circuit (EC) for a DNA/Au electrode, which was corroborated by electrochemical impedance spectroscopy (EIS) measurement. This model differs from the Randles circuit that is commonly used in double-layer simulations. The change in the potential relaxation and associated charge transfer resistance were used for sensing the DNA hybridization by using the redox pair Fe(CN)64-/Fe(CN)63+ as an electrochemical indicator. In order to determinate only the potential relaxation of the composed double layer, the faradic and double-layer current contributions were separated using a rectifier diode arrangement. A detection limit of 0.38 nM was obtained for the target HPV-16 DNA sequences. The biosensor showed a qualitative discrimination between a single-base mismatched sequence and the fully complementary HPV-16 DNA target. The results indicate that the discharge of the double-layer detection method can be used to develop an HPV DNA biosensor.

Highlights

The human papillomavirus (HPV) is one of the most common sexually transmitted infections, affecting the skin and mucous membranes
Conclusions on electrodes modified with DNA can be useful for the development of an Human Papillomavirus-16 (HPV-16) DNA biosensor
The relaxation variables following thethat hybridization process weredue change in maximum potential electrodes modified with can useful for ∆E

Summary

Introduction

The human papillomavirus (HPV) is one of the most common sexually transmitted infections, affecting the skin and mucous membranes. HPV has been detected in virtually all invasive cervical cancers and has been confirmed as the major cause of cervical cancer [1]. HPV is a group of non-enveloped, double-stranded DNA viruses composed of approximately 200 genotypes [2]. It has been shown that the 71.8% of worldwide invasive carcinomas are associated with high-risk HPV types (HR-HPV), mostly HPV-16 and HPV-18 [3]. The majority of human HR-HPV infections are cleared by the immune system within 1–2 years and are asymptomatic.

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