Abstract
Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects. Human gets exposure with genotoxins such as mycotoxins, polycyclic aromatic hydrocarbons, and n-nitroso-compounds through diet and environmental contaminations. Aflatoxin B1 is mycotoxin which was reported one of the most potent hepato-carcinogen and its exposure stems from human diet. In this study, we aimed to investigate DNA-bioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) cells against Aflatoxin B1 (AFB1) with comet assay without metabolic activation. The results showed that DNA-bioprotective effect of L. plantarum did reduce the AFB1’s genotoxic effect on colon adenocarcinoma (Caco-2) cells. Positive control (50 μM H2O2 applied) and high dose (>10ppm) AFB1 applied cells have the same comet tail appearance. Only visual scoring is performed. Besides negative control cells (Only PBS) and Lactobacillus plantarum+AFB1 mixture showed the same manner with each other; no comet tail detected. These results clearly indicate that L. plantarum is capable of reduce AFB1 safely without producing any by-products.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Current Research in Nutrition and Food Science Journal
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
