Abstract
The study of DNA interaction with the acetate bis(1,10-phenanthroline)silver(I) monohydrate in a solution is of interest both for understanding the mechanism of biological activity of silver compound and for forming ordered structures (DNA fibrils) that can be used to solve various problems in the field of nanotechnology. The analysis of changing the DNA conformation (secondary structure, persistent length and volume effects) during the interaction by the methods of UV spectroscopy with the analysis of DNA melting, circular dichroism, viscosity, flow birefringence, AFM (atomic force microscopy) and SEM (scanning electron microscopy) was performed. The formation of two types of complexes was observed. At lower concentration of compound in DNA solution, silver atoms form the coordination bonds with a macromolecule, while the released phenanthroline ligands intercalate between DNA bases. When the concentration of the compound increases, the phenanthroline ligands form an ordered “layer” around the helix. The excess of silver compounds in the DNA solution (with more than five silver atoms per base pair), DNA precipitation is observed with the formation of long fibrils. It was shown that the binding of silver to DNA during the formation of complexes provides further metallization of the resulting structures with the aid of reducing agents; phenanthroline ligands influence the result of such metallization.
Highlights
The usage of a DNA molecule to create various structures for nanoelectronics has become a fairly common technique
This study examines one of the ways to solve this problem by metallizing DNA through the reduction of silver after the formation of DNA complexes with silver compounds containing phenanthroline ligands
The hypochromic effect without shift of the DNA absorption band in complexes with Ag–Phen was observed at r ≤ 0.4, where r value indicates the number of silver atoms per one DNA base pair (Figure 2A)
Summary
The usage of a DNA molecule to create various structures for nanoelectronics has become a fairly common technique. There is often a need for DNA metallization. Natural DNA or synthetic polynucleotide chains are used for binding of modified metal nanoparticles, or, for example, as a template on which the reduction of pre-bound metal ions occurs [1,2,3,4]. This study examines one of the ways to solve this problem by metallizing DNA through the reduction of silver after the formation of DNA complexes with silver compounds containing phenanthroline ligands. The study of DNA interaction with the compound used in the research is of interest because of the biological activity of silver complexes with phenanthroline [5,6].
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