Abstract
The interaction of [Co(H2O)4(p-NO2C6H4COO)2]. 2H2O with sheep genomicDNA has been investigated by spectroscopic studies and electrophoresis measurements.The interaction between cobalt(II) p-nitrobenzoate and DNA has been followed by gelelectrophoresis while the concentration of the complex was increased from 0 to 14 mM.The spectroscopic study and electrophoretic experiments support the fact that the complexbinds to DNA by intercalation via p-nitrobenzoate into the base pairs of DNA. Themobility of the bands decreased as the concentration of complex was increased, indicatingthat there was increase in interaction between the metal ion and DNA.
Highlights
The interaction of transition metal complexes with DNA has been extensively studied in the past few years
The carcinogenicity of cobalt, nickel, cadmium, and arsenic compounds have been known for the long time
Even though only weakly mutagenic, several kinds of cellular damage have been identified, related with their carcinogenic potentials [1]. They were put in the induction of oxidative potential interactions with the removal of different kinds of DNA damage
Summary
The interaction of transition metal complexes with DNA has been extensively studied in the past few years. First indications that metal ions may diminish DNA repair processes were obtained by pronounced comutagenic effects of arsenic(III), cobalt(II), nickel(II) and cadmium(II) in bacteria as well as in mammalian cells. Based on these observations, detailed studies have been conducted to oxidative elucidate potential interactions with the removal of different types of DNA damage [1]. The binding properties to genomic DNA of this cobalt(II) complex, containing a ligand with nitro substituents at the 5-position of the phenyl group, was studied using absorption spectroscopy and electrophoreis measurements. These studies are necessary for the further comprehension of binding of transition metal complexes to DNA; they can serve as complementary studies for the corresponding ruthenium complexes
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